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Kingston Peptides
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Quality Assurance

Every peptide we sell undergoes rigorous analytical testing to ensure the highest standards of purity, identity, and consistency.

1

Solid-Phase Synthesis

Peptides are synthesised using Fmoc-based SPPS with automated synthesizers.

2

Purification

Reverse-phase HPLC purification removes truncated sequences and impurities.

3

Analytical Testing

HPLC purity analysis + mass spectrometry identity confirmation.

4

Lyophilisation

Freeze-dried for maximum stability and shipped with full COA.

HPLC Purity Analysis

High-Performance Liquid Chromatography (HPLC) separates and quantifies the peptide content of each batch. Our standard is >= 98% purity, verified by peak area integration. The resulting chromatogram is included in every Certificate of Analysis.

Grade HPLC Purity Use Case
Research ≥ 95% General in vitro studies
High-Purity ≥ 98% Quantitative assays
Ultra-Purity ≥ 99% Clinical standards

Mass Spectrometry Confirmation

Every batch is analysed by mass spectrometry (MS) to confirm the molecular weight of the synthesised peptide. The observed mass must match the theoretical mass within 1 Dalton. This ensures that the correct sequence was synthesised and that no major deletion peptides are present.

Residual Solvent Testing

After lyophilisation, trace solvents from synthesis remain. We test for and comply with ICH Q3C limits:

  • • Acetonitrile: < 410 ppm (Class 2)
  • • TFA (trifluoroacetic acid): < 250 ppm
  • • DMF (dimethylformamide): < 880 ppm

Certificate of Analysis (COA)

Every order includes a COA with HPLC chromatogram, MS data, and residual solvent levels. Verify any batch using our online tool.

Verify a Batch